Aquanaut Team
- Commander Nicholas J. M. Patrick, PhD
- Astronaut
Johnson Space Center - Satoshi Furukawa
- Astronaut
Japan Aerospace Exploration Agency - Christopher Gerty
- Constellation Program Aerospace Engineer
Johnson Space Center - Richard R. Arnold II
- Astronaut
Johnson Space Center - Jim Buckley
- Habitat Technician
- Dewey Smith
- Habitat Technician
Topside Support
- Bill Todd
- Project Lead
Johnson Space Center - Marc Reagan
- NEEMO Mission Director
- Monika K. Schultz
- Astronaut Expeditionary Training Manager
United Space Alliance - Daniel Sedej
- Chief of Systems Training
NASA, Johnson Space Center - Dr. Sam Strauss
- NASA Flight Surgeon
Johnson Space Center - Kevin Rullman
- NASA
Johnson Space Center - Ronald Stueber
- Engineering Staff IV
United Space Alliance - Karl Shreeves
Mission Physician
- Lt. Bradley Hickey
- Mission Physician
U.S. Navy